ANGPTL2 (Human) Recombinant Protein (Biotin)
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More Files
- More Functions
Surface Plasmon Resonance
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human A ngPTL2, His Tag with an affinity constant of 65.63 nM as determined in SPR assay (Biacore T200).
Tris-Bis PAGE
Biotinylated Human ANGPTL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human ANGPTL2 is greater than 95% as determined by SEC-HPLC.
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Specifications
Product Description
Human ANGPTL (Q9UKU9-1, Thr260-His493) partial recombinant protein with His-Avi tag at the C-Terminus expressed in HEK293 cells.
Sequence
Thr260-His493
Host
Human
Theoretical MW (kDa)
30.1
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The affinity constant was 65.63 nM, determined by SPR assay.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-Arginine, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Functional Study
Surface Plasmon Resonance
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human A ngPTL2, His Tag with an affinity constant of 65.63 nM as determined in SPR assay (Biacore T200). -
Gene Info — ANGPTL2
Entrez GeneID
23452Protein Accession#
Q9UKU9-1Gene Name
ANGPTL2
Gene Alias
ARP2, HARP, MGC8889
Gene Description
angiopoietin-like 2
Omim ID
605001Gene Ontology
HyperlinkGene Summary
Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. [provided by RefSeq
Other Designations
OTTHUMP00000022159|angiopoietin-related protein 2
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