ALCAM (Human) Recombinant Protein (Biotin)
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human ALCAM, His Tag at 1 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.18 ug/mL determined by ELISA.
Tris-Bis PAGE
Biotinylated Human ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human ALCAM is greater than 95% as determined by SEC-HPLC.
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Specifications
Product Description
Human ALCAM (Q13740-1, Trp28-Ala526) partial recombinant protein with His-Avi tag at the C-Terminus expressed in HEK293 cells.
Sequence
Trp28-Ala526
Host
Human
Theoretical MW (kDa)
58.9
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The EC50 was 0.18 ug/mL, measured by ELISA at 1 ug/mL.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human ALCAM, His Tag at 1 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.18 ug/mL determined by ELISA.Functional Study
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Gene Info — ALCAM
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Interactomes
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Pathways
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Diseases
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