

ANGPTL2 (Human) Recombinant Protein

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Specifications
Product Description
Human ANGPTL2 (Q9UKU9-1, Thr260-His493) partial recombinant protein with His-Avi tag at C-terminus expressed in HEK293 cells.
Sequence
Thr260-His493
Host
Human
Theoretical MW (kDa)
30.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Quality Control Testing
SEC-HPLC
SEC-HPLC
The purity of Human ANGPTL2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human ANGPTL2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SEC-HPLC
SPR
Tris-Bis PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from filtered solution in PBS, pH 7.4 (8% trehalose).
Storage Instruction
After reconstitution with deionized water to a final concentration more than 100 ug/ml, store at 4°C for 1 week. For long term storage, store at -80°C for 1 year.
Aliquot to avoid repeated freezing and thawing. -
Applications
Enzyme-linked Immunoabsorbent Assay
Functional Study
Surface Plasmon Resonance
Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Human ANGPTL2, His Tag with an affinity constant of 0.22 uM as determined in SPR assay (Biacore T200). -
Gene Info — ANGPTL2
Entrez GeneID
23452Protein Accession#
Q9UKU9-1Gene Name
ANGPTL2
Gene Alias
ARP2, HARP, MGC8889
Gene Description
angiopoietin-like 2
Omim ID
605001Gene Ontology
HyperlinkGene Summary
Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. [provided by RefSeq
Other Designations
OTTHUMP00000022159|angiopoietin-related protein 2
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