GFP monoclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of 293 cells transfected with a GFP construct. Using GFP monoclonal antibody (Cat # MAB8184).
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Specifications
Product Description
Mouse monoclonal antibody raised against full length recombinant GFP.
Immunogen
Recombinant protein corresponding to full length jellyfish GFP.
Host
Mouse
Reactivity
Jellyfishes
Specificity
This antibody detects GFP and its variants.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
Immunoprecipitation (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of 293 cells transfected with a GFP construct. Using GFP monoclonal antibody (Cat # MAB8184).Immunoprecipitation
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Publication Reference
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Transient decrease in F-actin may be necessary for translocation of proteins into dendritic spines.
Ouyang Y, Wong M, Capani F, Rensing N, Lee CS, Liu Q, Neusch C, Martone ME, Wu JY, Yamada K, Ellisman MH, Choi DW.
The European Journal of Neuroscience 2005 Dec; 22(12):2995.
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Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin.
Jurado C, Haserick JR, Lee J.
Molecular Biology of the Cell 2005 Feb; 16(2):507.
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A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype.
Wang L, Bhattacharyya N, Chelsea DM, Escobar PF, Banerjee S.
Cancer Research 2004 Nov; 64(21):7673.
Application：IP-WB, Human , HEK 293 cells.
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Transient decrease in F-actin may be necessary for translocation of proteins into dendritic spines.
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