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  • Publish: 2014/4/15
  • Time: 2:27
With the aid of micromanipulator, precise physical interactions under microscopic level becomes achievable. In concert with hydraulic drive systems, it is principally the glass probe that makes the physical contack with target cell for purposes like single cell collection, injection, patch clamping, etc. Here is the presentation of a typical procedure for probe preparation, including steps of glass capillary pulling and micropipette tip processing. Instruments adopted here are MF-900 microforge and PC-10 puller, both from NARISHIGE Group.
TOP 10
  1. Western Blot
  2. Real-time PCR
  3. Reverse Transcription PCR
  4. Immunohistochemistry
  5. Indirect ELISA
  6. Polymerase Chain Reaction
  7. H&E Staining
  8. Cell Culture (Attached Cell)
  9. Immunofluorescence
  10. Competitive ELISA
  • List alphabetically by video name :
  • Result: 1 - 11
  • Pages:  1 
   
  • The Ciliopathy-associated Protein Homologs RPGRIP1

    The aim of this study was to gain more insight in the function of two ciliopathy-associated protein homologs, RPGR interacting protein 1 (RPGRIP1), and RPGRIP1-like protein (RPGRIP1L). Base on the data, the authors hypothesize that RPGRIP1 and RPGRIP1L function as cilium-specific scaffolds that recruit a Nek4 signaling network which regulates cilium stability.

    Publish: 2011/8/30

  • Tissue Grinder

    The tissue grinder provides rapid and efficient disruption of up to 12 biological samples, including animal and human tissues, plant tissues, bacteria, and yeast. Disruption and homogenization are achieved through the beating and grinding effect of beads on the sample material as they are shaken together in 2 ml sample tubes.

    Publish: 2010/11/26

  • Transfected Lysate (Native)

    Transfected lysate is useful as a positive control in Western Blot. The full-length protein construct is expressed in HEK293T cells and prepared as ready-to-use, native lysate in modified RIPA Buffer.

    Publish: 2010/1/29

  • Terrific Broth (TB) Preparation

    Terrific Broth (TB) is an enriched medium used with glycerol in cultivating Escherichia coli. TB allows the bacteria to produce a greater number of recombinant proteins and plasmid yield. This video shows you how to prepare TB.

    Publish: 2011/7/1

  • Total IgG (Human) ELISA Kit

    Total IgG (Human) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative detection of total human IgG in human serum and plasma. This video shows the procedures of the kit.

    Publish: 2014/4/1

  • TSB Medium Preparation

    Tryptic Soy Broth (TSB) is a general purpose culture broth used to support the growth of a wide variety of aerobic, facultative and anaerobic bacteria. This video demonstrates the steps to prepare TSB medium.

    Publish: 2011/8/2

  • Tissue Lysate Preparation (Native)

    This protocol involves three steps to prepare tissue lysate. First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as read-to-use, native lysate in modified RIPA Buffer.

    Publish: 2010/6/7

  • Transfected Lysate (Denatured)

    Transfected lysate is useful as a positive control in Western Blot. The full-length protein construct is expressed in HEK293T cells and prepared as ready-to-use, denatured lysate in 1x Sample Buffer.

    Publish: 2010/1/29

  • Tissue Embedding

    Tissue embedding is a tissue preparation technique. The tissue samples are embedded in embedding material (such as agar, gelatine, or wax) and ready to be sliced. This video describes how tissue embedding.

    Publish: 2011/8/30

  • Tissue Microarray

    Tissue Microarray is a powerful new technology for high throughput analysis of protein expression in a large number of tissue samples. Hundreds of tissue cores are arranged on a single slide, and then analyzed by immunohistochemistry staining. We offer a large collection of immmunohistochmistry (IHC) validated antibodies for the tissue microarray platform.

    Publish: 2009/12/8

  • Tissue Lysate Preparation (Denatured)

    This protocol involves three steps to prepare tissue lysate. First, the tissue is grinded. Then, the cells are lysed in the lysis buffer. After ultra speed centrifuge, tissue lysate is prepared as read-to-use, denatured lysate in 1x Sample Buffer.

    Publish: 2010/6/7

  • Result: 1 - 11
  • Pages:  1 
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