The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology. This video shows how to extract total RNA from tissue.
Rotary microtome is an instrument used to cut the blocks of tissue embedded in paraffin into micro fine slices (known as sections). Microtome is an important device in microscopy preparation, allowing for the preparation of samples for observation under transmitted light or electron radiation.
RNA electrophoresis is an analytical technique used to separate RNA fragments. RNA has the tendency to form both secondary and tertiary structures that can impede its separation. So a denaturing condition is performed in formaldehyde and MOPS buffer system. RNA under this condition is fully denatured and migrates according to its molecular weight.
FUJIFILM FLA-2000 is a multiple imaging application suitable for fluorescence and radioisotopic measurement. Laser scanning with the FLA-2000, instead of using conventional photography, permits analysis and digital storage. With FUJIFILM Imaging Plate (IP), a technology which is 100 times more sensitive to radiation than X-ray film, exposure times are reduced to 1/10 - 1/20 of film and amount of radioisotope is reduced.
Reverse transcription PCR includes two steps. The first step is reverse transcription, in which RNA is reverse transcribed to its DNA complement (complementary DNA, or cDNA) using reverse transcriptase and primers. The second step is amplification using traditional or real-time PCR.
Real-time PCR, also called quantitative real time PCR (Q-PCR/qPCR), is used to amplify and simultaneously quantify a targeted DNA molecule. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample.
The RNA extraction procedure (STRATAGENE) represents a well-established technology for RNA purification. This technology combines the selective binding properties of a silica-based membrane with the speed of microspin technology. This video shows how to extract total RNA from cell culture.
RNA interference is a gene-silencing technique used in studying the absence of normal gene action. To better understand its function and role in disease, you can transfect siRNA into cell line to turn gene expression off or knock it down.
TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.
Agarose gel electrophoresis is a method used to separate a mixed population of DNA or RNA fragments by length. This video shows you how to prepare the formaldehyde agarose gel for the electrophoresis of RNA.