Proteins with histidine tag can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This video provides generic protocol to purify 6xHis-tagged protein by Nickel nitrilotriacetic (Ni-NTA) sepharose.
The Human Protein Reference Database represents a centralized platform to visually depict and integrate information pertaining to domain architecture, post-translational modifications, interaction networks and disease association for each protein in the human proteome.
Handling of adhering cell is the key point before culturing. This video shows you how to disinfect the flask well, confirm the condition of the cells under microscopy, and re-attach the cell that may have detached during transportation.
Handling of suspending cell is the key point before culturing. This video shows you how to disinfect the tube well, transfer the cell suspension to a flask or dish, and make sure the condition of the cells under microscopy.
H&E stain is a popular staining method in histology. Its a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you will usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink).
Human embryonic stem cells (hESCs) are pluripotent cells are able to differentiate into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. This video describes how a vial of cryopreserved hESCs is thawed and seeded on a feeder layer of inactivated Mouse Embryonic Fibroblasts (MEFs).