Map Viewer allows you to view and search complete genome of an organism, display chromosome maps, and zoom into progressively greater levels of detail, down to the sequence data for a region of interest.
Gel extraction is a technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis. Three basic steps are involved: slicing the bands of interest on UV light exposure, isolating the DNA from those bands, and removing the accompanying salts and stain.
Conserved domains database (CDD) is a protein annotation resource that consists of a collection of well-annotated multiple sequence alignment models for ancient domains and full-length proteins. These are available as position-specific score matrices (PSSMs) for fast identification of conserved domains in protein sequences via RPS-BLAST (Reverse Position-Specific).
The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. In contrast, Gateway recombination cloning uses a one hour, 99%-efficient, reversible recombination reaction, without using restriction enzymes, ligase, and subcloning step. It is an extremely fast and efficient way to clone your gene.
HomoloGene database identifies homologs among the annotated genes of more than a dozen completely sequenced eukaryotic genomes using an automated procedure. It can find gene homologs (paralogs, orthologs, homologs) based on protein sequence similarity and access pre-computed multiple alignments of homologous proteins.
Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. We use GST-fusion protein to purify and detect proteins of interest. The GST-fusion protein can be purified from cells via its high affinity for glutathione.
Gelatin solution is used to coat 6-well plates for culture of Mouse Embryonic Fibroblasts (MEFs). MEFs may be used as feeder cell layer to support the survival and growth of human embryonic stem cells (hESCs). This video shows the steps for preparation of gelatin solution and gelatin-coated plates.
Gene pathway represents molecular pathways for metabolism, genetic information processing, environmental information processing, other cellular processes, human diseases, and drug development.
GST fusion proteins allow convenient affinity purification of many proteins of interest but the GST-tag may become inconvenient in various downstream applications. We use PreScission protease to digest the GST fusion proteins and the protein without GST-tag is obtained.
GeneCards® is an integrated database of human genes that includes automatically-mined genomic, proteomic and transcriptomic information, as well as orthologies, disease relationships, SNPs, gene expression, gene function, and service links for ordering assays and antibodies.
The Reference Sequence (RefSeq) database is a non-redundant collection of richly annotated DNA, RNA, and protein sequences from diverse taxa. RefSeq also reports the information about exon and intron boundaries and length.
ORF Finder identifies all possible ORFs in a DNA sequence by locating the standard and alternative stop and start codons. The deduced amino acid sequences can then be used to BLAST against GenBank.
The Gene Expression Omnibus (GEO) is a public repository that stores original submitter-supplied curated gene expression DataSets. This video shows you how to enter search terms to locate experiments of interest and interpret GEO DataSets results pages.
dbSNP resource serves both as a repository for genomic variation data (including single nucleotide polymorphisms, microsatellites and small insertion/deletion mutations) and as a computational analysis resource. dbSNP data will provide the researcher with extensive data and information about variations, evolution, disease and more.