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AbVideo™ - Cre-Lox Recombination

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  • Publish: 2010/4/12
  • Time: 4:10
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by Abnova  


Cre-Lox recombination is a special type of site-specific recombination. The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur. It is often used in the generation of knockout and conditional knockout animals.


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  • Gateway Recombination

    The typical cloning workflow involves many steps, particularly, traditional restriction enzyme cloning. In contrast, Gateway recombination cloning uses a one hour, 99%-efficient, reversible recombination reaction, without using restriction enzymes, ligase, and subcloning step. It is an extremely fast and efficient way to clone your gene.

    Publish: 2010/4/12

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