The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions (e.g. protein dimerization) and modifications (e.g. protein phosphorylation) in tissue and cell samples prepared for microscopy. Each detected signal is visualized as an individual fluorescent dot, these signals can be quantified (counted) and assigned to a specific subcellular location based on microscopy images. This revolutionary technique enables unprecedented specificity and sensitivity of protein detection and quantification for high performance immunofluorescence and immunohistochemistry applications. Leveraging on Abnova's manufacturing capability and growing collection of 50,000+ antibodies, hundreds of target specific and validated assays are being developed in cells and tissue samples.
Principle of Proximity Ligation Assay
Incubate with target primary antibodies from two different species
Hybridize connector oligos
Ligation to form a complete DNA circle
Rolling circle amplification
Add fluorescent probes to reveal protein protein interaction