AKT2 (phospho S474) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Phospho-AKT2 Ser474 polyclonal antibody. Western blot analysis of AKT2 expression in human kidney.
Western Blot (Cell lysate)
Phospho-AKT2 Ser474 polyclonal antibody. Western blot analysis of AKT2 expression in Jurkat.
Western Blot (Cell lysate)
Western blot analysis of extract from A2780 cells using AKT2 (phospho S474) polyclonal antibody (Cat # PAB8183).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AKT2 (phospho S474) polyclonal antibody (Cat # PAB8183).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S474 of human AKT2.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Specificity
Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474.
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Phospho-AKT2 Ser474 polyclonal antibody. Western blot analysis of AKT2 expression in human kidney.Western Blot (Cell lysate)
Phospho-AKT2 Ser474 polyclonal antibody. Western blot analysis of AKT2 expression in Jurkat.Western Blot (Cell lysate)
Western blot analysis of extract from A2780 cells using AKT2 (phospho S474) polyclonal antibody (Cat # PAB8183).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AKT2 (phospho S474) polyclonal antibody (Cat # PAB8183). -
Gene Info — AKT2
Entrez GeneID
208Gene Name
AKT2
Gene Alias
PKBB, PKBBETA, PRKBB, RAC-BETA
Gene Description
v-akt murine thymoma viral oncogene homolog 2
Gene Ontology
HyperlinkGene Summary
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations
Murine thymoma viral (v-akt) homolog-2|rac protein kinase beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats.
Gezginci-Oktayoglu S, Sacan O, Bolkent S, Ipci Y, Kabasakal L, Sener G, Yanardag R.
Acta Histochemica 2014 Jan; 116(1):32.
Application：IS, Rat, Liver.
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Chard (Beta vulgaris L. var. cicla) extract ameliorates hyperglycemia by increasing GLUT2 through Akt2 and antioxidant defense in the liver of rats.
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