ACTB polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of mouse C2C12 cells untreated (lane 1), or treated with pervanadate (1 mM) for 30 min (lane 2). The blot was probed with ACTB polyclonal antibody (Cat # PAB7880).
Immunofluorescence
Immunocytochemical labeling using ACTB polyclonal antibody (Cat # PAB7880) in C2C12 cells untreated (control) or treated with pervanadate (1 mM) for 30 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ACTB.
Host
Rabbit
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This sequence is identical to similar regions in all four a actins, as well as in g actin, and is well conserved in actins from most eukaryotic species.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of mouse C2C12 cells untreated (lane 1), or treated with pervanadate (1 mM) for 30 min (lane 2). The blot was probed with ACTB polyclonal antibody (Cat # PAB7880).Immunofluorescence
Immunocytochemical labeling using ACTB polyclonal antibody (Cat # PAB7880) in C2C12 cells untreated (control) or treated with pervanadate (1 mM) for 30 min.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Publication Reference
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Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments.
Liu X, Shu S, Hong MS, Levine RL, Korn ED.
PNAS 2006 Sep; 103(37):13694.
Application：WB, Recombinant protein.
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Actin-binding proteins.
Winder SJ, Ayscough KR.
Journal of Cell Science 2005 Feb; 118(Pt 4):651.
Application：WB, Human, Mammalian cells.
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Actin tyrosine dephosphorylation by the Src homology 1-containing protein tyrosine phosphatase is essential for actin depolymerization after membrane IgM cross-linking.
Baba T, Fusaki N, Shinya N, Iwamatsu A, Hozumi N.
The Journal of Immunology 2003 Apr; 170(7):3762.
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Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments.
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