CD14 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
CD14 polyclonal antibody (Cat # PAB6955) (2 ug/mL) staining of Human Lymph node (1) + peptide (2) and Tonsil (3) + peptide (4) lysate (35 ug protein in RIPA buffer) Detected by chemiluminescence.
Flow Cytometry
CD14 polyclonal antibody (Cat # PAB6955) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CD14.
Immunogen
A synthetic peptide corresponding to human CD14.
Sequence
C-KRVDADADPRQYAD
Host
Goat
Theoretical MW (kDa)
40.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Flow Cytometry (10 ug/mL)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CD14 polyclonal antibody (Cat # PAB6955) (2 ug/mL) staining of Human Lymph node (1) + peptide (2) and Tonsil (3) + peptide (4) lysate (35 ug protein in RIPA buffer) Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
CD14 polyclonal antibody (Cat # PAB6955) Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — CD14
Entrez GeneID
929Protein Accession#
NP_000582.1Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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Publication Reference
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The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.
Biochemical and Biophysical Research Communications 2005 Mar; 328(1):173.
Application：Blocking, Human, Recombinant proteins.
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The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
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