FCGR2B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
FCGR2B polyclonal antibody (Cat # PAB6767) (0.3 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing FCGR2B and probed with FCGR2B polyclonal antibody (Cat # PAB6767) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FCGR2B polyclonal antibody (Cat # PAB6767) (8 ug/mL) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FCGR2B.
Immunogen
A synthetic peptide corresponding to C-terminus of human FCGR2B.
Sequence
C-PDALEEPDDQNRI
Host
Goat
Theoretical MW (kDa)
34
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (6-8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
FCGR2B polyclonal antibody (Cat # PAB6767) (0.3 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing FCGR2B and probed with FCGR2B polyclonal antibody (Cat # PAB6767) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FCGR2B polyclonal antibody (Cat # PAB6767) (8 ug/mL) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FCGR2B
Entrez GeneID
2213Protein Accession#
NP_003992.3;NP_001002273.1;NP_001002274.1;NP_001002275.1;NP_001177757.1Gene Name
FCGR2B
Gene Alias
CD32, CD32B, FCG2, FCGR2, IGFR2
Gene Description
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Gene Ontology
HyperlinkGene Summary
low affinity IIb
Other Designations
Fc fragment of IgG, low affinity II, receptor for (CD32)|Fc fragment of IgG, low affinity IIb, receptor for (CD32)|Fc gamma RIIb|OTTHUMP00000032379
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Interactome
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Pathway
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Disease
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Publication Reference
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Restoration of tolerance in lupus by targeted inhibitory receptor expression.
McGaha TL, Sorrentino B, Ravetch JV.
Science 2005 Jan; 307(5709):590.
Application：Flow Cyt, Mouse , Mouse B cells.
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Restoration of tolerance in lupus by targeted inhibitory receptor expression.
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