TFPI polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
TFPI polyclonal antibody (Cat # PAB6017) staining (0.3 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing TFPI and probed with TFPI polyclonal antibody (Cat # PAB6017) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TFPI.
Immunogen
A synthetic peptide corresponding to human TFPI.
Sequence
C-VKIAYEEIFVKNM
Host
Goat
Theoretical MW (kDa)
35
Reactivity
Human
Specificity
This antibody is expected to recognize isoform a (NP_006278.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TFPI polyclonal antibody (Cat # PAB6017) staining (0.3 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing TFPI and probed with TFPI polyclonal antibody (Cat # PAB6017) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TFPI
Entrez GeneID
7035Protein Accession#
NP_006278.1Gene Name
TFPI
Gene Alias
EPI, LACI, TFI, TFPI1
Gene Description
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Omim ID
152310Gene Ontology
HyperlinkGene Summary
This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq
Other Designations
OTTHUMP00000163472|anti-convertin|extrinsic pathway inhibitor|lipoprotein-associated coagulation inhibitor|tissue factor pathway inhibitor
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Interactome
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Pathway
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Disease
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Publication Reference
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Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains.
Wun TC, Kretzmer KK, Girard TJ, Miletich JP, Broze GJ Jr.
The Journal of Biological Chemistry 1988 May; 263(13):6001.
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Cloning and characterization of a cDNA coding for the lipoprotein-associated coagulation inhibitor shows that it consists of three tandem Kunitz-type inhibitory domains.
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