SOX2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of SOX2 polyclonal antibody (Cat # PAB3876) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of SOX2 (arrow) using rabbit SOX2 polyclonal antibody (Cat # PAB3876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 polyclonal antibody (Cat # PAB3876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence
Immunofluorescence analysis of SOX2 polyclonal antibody (Cat # PAB3876) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using SOX2 polyclonal antibody (Cat # PAB3876)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SOX2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SOX2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Flow Cytometry (1:10-50)
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SOX2 polyclonal antibody (Cat # PAB3876) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified polyclonal antibody.Western Blot (Transfected lysate)
Western blot analysis of SOX2 (arrow) using rabbit SOX2 polyclonal antibody (Cat # PAB3876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 polyclonal antibody (Cat # PAB3876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunofluorescence
Immunofluorescence analysis of SOX2 polyclonal antibody (Cat # PAB3876) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using SOX2 polyclonal antibody (Cat # PAB3876)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — SOX2
Entrez GeneID
6657Protein Accession#
NP_003097;P48431Gene Name
SOX2
Gene Alias
ANOP3, MCOPS3, MGC2413
Gene Description
SRY (sex determining region Y)-box 2
Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
Other Designations
SRY-related HMG-box gene 2|sex-determining region Y-box 2|transcription factor SOX2
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Interactome
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Disease
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Publication Reference
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Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M.
Genes & Development 2003 Aug; 17(16):2048.
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POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors.
Looijenga LH, Stoop H, de Leeuw HP, de Gouveia Brazao CA, Gillis AJ, van Roozendaal KE, van Zoelen EJ, Weber RF, Wolffenbuttel KP, van Dekken H, Honecker F, Bokemeyer C, Perlman EJ, Schneider DT, Kononen J, Sauter G, Oosterhuis JW.
Cancer Research 2003 May; 63(9):2244.
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Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.
Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, Smith A.
Cell 1998 Oct; 95(3):379.
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Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers.
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