AKT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1 : Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423056) with AKT1 polyclonal antibody (Cat # PAB28728) at 1:100-1:250 dilution.
Immunohistochemistry
Immunohistochemical staining of human gall bladder with AKT1 polyclonal antibody (Cat # PAB28728) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with AKT1 polyclonal antibody (Cat # PAB28728) at 1-4 ug/mL shows positivity in nucleus but not nucleoli and cytoskeleton (microtubules).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant AKT1.
Immunogen
Recombinant protein corresponding to amino acids of human AKT1.
Sequence
ERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1-4 ug/ml)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1 : Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423056) with AKT1 polyclonal antibody (Cat # PAB28728) at 1:100-1:250 dilution.Immunohistochemistry
Immunohistochemical staining of human gall bladder with AKT1 polyclonal antibody (Cat # PAB28728) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with AKT1 polyclonal antibody (Cat # PAB28728) at 1-4 ug/mL shows positivity in nucleus but not nucleoli and cytoskeleton (microtubules). -
Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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