ATF2 (phospho T53/T71) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells using ATF2 (phospho T53/T71) polyclonal antibody (Cat # PAB25252).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (phospho T53/T71) polyclonal antibody(Cat # PAB25252).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ATF2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T71/T53 of human ATF2.
Sequence
T-P-Tp-P-T
Host
Rabbit
Theoretical MW (kDa)
65-75
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells using ATF2 (phospho T53/T71) polyclonal antibody (Cat # PAB25252).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2 (phospho T53/T71) polyclonal antibody(Cat # PAB25252). -
Gene Info — ATF2
Entrez GeneID
1386Protein Accession#
P15336Gene Name
ATF2
Gene Alias
CRE-BP1, CREB2, HB16, MGC111558, TREB7
Gene Description
activating transcription factor 2
Omim ID
123811Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000163262|activating transcription factor 2 splice variant ATF2-var2|cAMP responsive element binding protein 2, formerly
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Interactome
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Pathway
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Disease
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