EPOR polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPOR.
Immunogen
A synthetic peptide corresponding to residues surrounding Y368 of human EPOR.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to EPOR.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells, treated with EPO (20 U/mL, 15 mins), using EPOR polyclonal antibody (Cat # PAB18350).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using EPOR polyclonal antibody (Cat # PAB18350).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPOR
Entrez GeneID
2057Protein Accession#
P19235Gene Name
EPOR
Gene Alias
MGC138358
Gene Description
erythropoietin receptor
Gene Ontology
HyperlinkGene Summary
The erythropoietin receptor is a member of the cytokine receptor family. Upon erythropoietin binding, the erythropoietin receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. [provided by RefSeq
Other Designations
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Interactome
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