ACTB polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide corresponding to human ACTB.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to ACTB.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
ELISA(1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using ACTB polyclonal antibody (Cat # PAB18050).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ACTB polyclonal antibody (Cat # PAB18050).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Disease
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Publication Reference
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Suprabasal Dsg2 expression in transgenic mouse skin confers a hyperproliferative and apoptosis-resistant phenotype to keratinocytes.
Brennan D, Hu Y, Joubeh S, Choi YW, Whitaker-Menezes D, O'Brien T, Uitto J, Rodeck U, Mahoney MG.
Journal of Cell Science 2007 Mar; 120(Pt 5):758.
Application:WB-Ti, Mouse , Mouse skin extracts.
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Immunization with murine breast cancer cells treated with antisense oligodeoxynucleotides to type I insulin-like growth factor receptor induced an antitumoral effect mediated by a CD8+ response involving Fas/Fas ligand cytotoxic pathway.
Schillaci R, Salatino M, Cassataro J, Proietti CJ, Giambartolomei GH, Rivas MA, Carnevale RP, Charreau EH, Elizalde PV.
The Journal of Immunology 2006 Mar; 176(6):3426.
Application:Wb, Mouse, C4HD cells.
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Identification and characterization of two novel splicing isoforms of human estrogen-related receptor beta.
Zhou W, Liu Z, Wu J, Liu JH, Hyder SM, Antoniou E, Lubahn DB.
The Journal of Clinical Endocrinology and Metabolism 2006 Feb; 91(2):569.
Application:WB, Human, C4-12-5, Ishikawa cells, Kidney tissues.
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Regulation of microfilament organization by Kaposi sarcoma-associated herpes virus-cyclin.CDK6 phosphorylation of caldesmon.
Cuomo ME, Knebel A, Platt G, Morrice N, Cohen P, Mittnacht S.
The Journal of Biological Chemistry 2005 Oct; 280(43):35844.
Application:WB-Re, Recombinant protein.
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Suprabasal Dsg2 expression in transgenic mouse skin confers a hyperproliferative and apoptosis-resistant phenotype to keratinocytes.
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