MMP2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The protein derived from the LoVo whole cell lysate (Lane 1) or the concentrated serum free culture medium of LoVo were resolved onto 10% SDS-PAGE, transferred to NC membrane, followed by an immunoblotting with MMP2 polyclonal antibody (Cat # PAB12711) at 1 : 1000.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta stained with MMP2 polyclonal antibody (Cat # PAB12711) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP2.
Immunogen
A synthetic peptide corresponding to C-terminus of human MMP2.
Host
Rabbit
Theoretical MW (kDa)
62, 72
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~62 and 72 KDa of human MMP2 protein.
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (0.01-0.1 ug/mL)
Immunohistochemistry (2-5 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.1-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (antibody stabilizer).
Storage Instruction
Store at 4°C for three month. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The protein derived from the LoVo whole cell lysate (Lane 1) or the concentrated serum free culture medium of LoVo were resolved onto 10% SDS-PAGE, transferred to NC membrane, followed by an immunoblotting with MMP2 polyclonal antibody (Cat # PAB12711) at 1 : 1000.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta stained with MMP2 polyclonal antibody (Cat # PAB12711) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MMP2
Entrez GeneID
4313Gene Name
MMP2
Gene Alias
CLG4, CLG4A, MMP-II, MONA, TBE-1
Gene Description
matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
collagenase type IV-A|matrix metalloproteinase 2|matrix metalloproteinase-II|neutrophil gelatinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Matrix metalloproteinases MMP2 and MMP9 are produced in early stages of kidney morphogenesis but only MMP9 is required for renal organogenesis in vitro.
Lelongt B, Trugnan G, Murphy G, Ronco PM.
The Journal of Cell Biology 1997 Mar; 136(6):1363.
Application：IF, WB-Ce, Mouse, Macrophage, NIH/3T3 cells, Kidney.
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Matrix metalloproteinases MMP2 and MMP9 are produced in early stages of kidney morphogenesis but only MMP9 is required for renal organogenesis in vitro.
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