MYC (phospho T58) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of MYC (phospho T58) polyclonal antibody (Cat # PAB0541) in human TPA activated HeLa cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb.
(0: without TPA; 1: 60 ug/ml TPA, 15 min; 2: 60 ug/ml TPA, 30 min; 3: 60 ug/ml TPA, 45 min; 4: 125 ug/ml TPA, 15 min; 5: 125 ug/ml TPA, 30 min; 6: 125 ug/ml TPA, 45 min; 7: 250 ug/ml TPA, 15 min; 8: 250 ug/ml TPA, 30 min; 9: 250 ug/ml, 45 min).
Dot Blot (Peptide)
Dot blot analysis of MYC (phospho T58) polyclonal antibody (Cat # PAB0541) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYC.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T58 of human MYC.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Dot Blot (1:100-500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MYC (phospho T58) polyclonal antibody (Cat # PAB0541) in human TPA activated HeLa cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb.
(0: without TPA; 1: 60 ug/ml TPA, 15 min; 2: 60 ug/ml TPA, 30 min; 3: 60 ug/ml TPA, 45 min; 4: 125 ug/ml TPA, 15 min; 5: 125 ug/ml TPA, 30 min; 6: 125 ug/ml TPA, 45 min; 7: 250 ug/ml TPA, 15 min; 8: 250 ug/ml TPA, 30 min; 9: 250 ug/ml, 45 min).Dot Blot (Peptide)
Dot blot analysis of MYC (phospho T58) polyclonal antibody (Cat # PAB0541) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — MYC
Entrez GeneID
4609Protein Accession#
NP_002458;P01106Gene Name
MYC
Gene Alias
bHLHe39, c-Myc
Gene Description
v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
Other Designations
avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|v-myc avian myelocytomatosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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A Comparison of Phosphospecific Affinity Reagents Reveals the Utility of Recombinant Forkhead-associated Domains in Recognizing Phosphothreonine-Containing Peptides.
Venegas LA, Pershad K, Bankole O, Shah N, Kay BK.
New Biotechnology 2016 Sep; 33(5 Pt A):537.
Application：ELISA, Synthesized Peptides.
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Prolactin induces c-Myc expression and cell survival through activation of Src/Akt pathway in lymphoid cells.
Dominguez-Caceres MA, Garcia-Martinez JM, Calcabrini A, Gonzalez L, Porque PG, Leon J, Martin-Perez J.
Oncogene 2004 Sep; 23(44):7378.
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p19ARF directly and differentially controls the functions of c-Myc independently of p53.
Qi Y, Gregory MA, Li Z, Brousal JP, West K, Hann SR.
Nature 2004 Oct; 431(7009):712.
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Level of MYC overexpression in pediatric Burkitt's lymphoma is strongly dependent on genomic breakpoint location within the MYC locus.
Wilda M, Busch K, Klose I, Keller T, Woessmann W, Kreuder J, Harbott J, Borkhardt A.
Genes, Chromosomes & Cancer 2004 Oct; 4(2):178.
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A Comparison of Phosphospecific Affinity Reagents Reveals the Utility of Recombinant Forkhead-associated Domains in Recognizing Phosphothreonine-Containing Peptides.
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