CD44 monoclonal antibody, clone 5C10
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of Recombinant human CD44 protein.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CD44 monoclonal antibody, clone 5C10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells. The cell was stained with CD44 monoclonal antibody, clone 5C10 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CD44.
Immunogen
Recombinant protein corresponding to amino acids 21-145 of human CD44.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of Recombinant human CD44 protein.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with CD44 monoclonal antibody, clone 5C10 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells. The cell was stained with CD44 monoclonal antibody, clone 5C10 at 2-5 ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). -
Gene Info — CD44
Entrez GeneID
960Protein Accession#
P16070Gene Name
CD44
Gene Alias
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Gene Description
CD44 molecule (Indian blood group)
Omim ID
107269Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations
CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl
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Interactome
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Pathway
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Disease
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Publication Reference
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The liberation of CD44.
Cichy J, Puré E.
The Journal of Cell Biology 2003 Jun; 161(5):839.
Application：Flow Cyt, Func, Human, Mouse, Mammalian cells.
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The normal structure and function of CD44 and its role in neoplasia.
Sneath RJ, Mangham DC.
Molecular Pathology 1998 Aug; 51(4):191.
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The liberation of CD44.
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