EGFR (phospho Y1101) monoclonal antibody, clone M199
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More Files
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Western Blot (Cell lysate)
Western blot of human A-431 cells untreated (lane 1) or treated with EGF (100 ng/ml) for 5 min (lane 2). The blot was probed with EGFR (phospho Y1101) monoclonal antibody, clone M199 (Cat # MAB1382).
Immunofluorescence
Immunocytochemical labeling in A-431 cells untreated (1) or treated with EGF (100 ng/ml) for 5 min (2). The cells were labeled EGFR (phospho Y1101) monoclonal antibody, clone M199 (Cat # MAB1382).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of EGFR.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1101 of human EGFR.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This human EGFR sequence has high homology to rat and mouse EGFR, and is not conserved in other ErbB family members.
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Immunocytochemistry (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of human A-431 cells untreated (lane 1) or treated with EGF (100 ng/ml) for 5 min (lane 2). The blot was probed with EGFR (phospho Y1101) monoclonal antibody, clone M199 (Cat # MAB1382).Immunocytochemistry
Immunofluorescence
Immunocytochemical labeling in A-431 cells untreated (1) or treated with EGF (100 ng/ml) for 5 min (2). The cells were labeled EGFR (phospho Y1101) monoclonal antibody, clone M199 (Cat # MAB1382).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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LRIG2 is a growth suppressor of Hec-1A and Ishikawa endometrial adenocarcinoma cells by regulating PI3K/AKT- and EGFR-mediated apoptosis and cell-cycle.
Suh DS, Park SE, Jin H, Lee K, Bae J.
Oncogenesis 2018 Jan; 7(1):3.
Application：WB-Ce, Human, Hec-1A cells.
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Systematic analysis of the epidermal growth factor receptor by mass spectrometry reveals stimulation-dependent multisite phosphorylation.
Boeri Erba E, Bergatto E, Cabodi S, Silengo L, Tarone G, Defilippi P, Jensen ON.
Molecular & Cellular Proteomics 2005 May; 4(8):1107.
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The EGF receptor: a nexus for trafficking and signaling.
Carpenter G.
Bioessays 2000 Aug; 22(8):697.
Application：IHC, WB-Tr, Human, Rat, A-431, PC-12 cells, Mammalian cells.
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LRIG2 is a growth suppressor of Hec-1A and Ishikawa endometrial adenocarcinoma cells by regulating PI3K/AKT- and EGFR-mediated apoptosis and cell-cycle.
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