AKT1 (phospho T308) monoclonal antibody, clone 18F3.H11
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of unstimulated (Lane 1) and PDGF stimulated (Lane 2) NIH/3T3 cell lysates with AKT1 (phospho T308) monoclonal antibody, clone 18F3.H11 (Cat # MAB10260) at 1:4000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human brain cerebellum with AKT1 (phospho T308) monoclonal antibody, clone 18F3.H11 (Cat # MAB10260) at 20 ug/mL dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of AKT1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T308 of human AKT1.
Host
Mouse
Theoretical MW (kDa)
56
Reactivity
Human, Mouse
Specificity
This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur.
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:20000)
Western Blot (1:500-1:3000)
Immunohistochemistry (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of unstimulated (Lane 1) and PDGF stimulated (Lane 2) NIH/3T3 cell lysates with AKT1 (phospho T308) monoclonal antibody, clone 18F3.H11 (Cat # MAB10260) at 1:4000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed paraffin-embedded human brain cerebellum with AKT1 (phospho T308) monoclonal antibody, clone 18F3.H11 (Cat # MAB10260) at 20 ug/mL dilution.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Interactome
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Pathway
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Disease
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