QPRT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
QPRT transfected lysate.
Western Blot
Lane 1: QPRT transfected lysate ( 30.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-QPRT full-length
Host
Human
Theoretical MW (kDa)
30.8
Interspecies Antigen Sequence
Mouse (84); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-QPRT antibody (H00023475-B04) by Western Blots.
SDS-PAGE Gel
QPRT transfected lysate.
Western Blot
Lane 1: QPRT transfected lysate ( 30.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — QPRT
Entrez GeneID
23475GeneBank Accession#
NM_014298.3Protein Accession#
NP_055113.2Gene Name
QPRT
Gene Alias
QPRTase
Gene Description
quinolinate phosphoribosyltransferase
Omim ID
606248Gene Ontology
HyperlinkGene Summary
This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. [provided by RefSeq
Other Designations
nicotinate-nucleotide pyrophosphorylase (carboxylating)
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Interactome
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Pathway
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