TRIM23 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: TRIM23 transfected lysate ( 64.1 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
TRIM23 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRIM23 full-length
Host
Human
Theoretical MW (kDa)
63.25
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRIM23 antibody (H00000373-B01) by Western Blots.
Western Blot
Lane 1: TRIM23 transfected lysate ( 64.1 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
TRIM23 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRIM23
Entrez GeneID
373GeneBank Accession#
NM_001656Protein Accession#
NP_001647Gene Name
TRIM23
Gene Alias
ARD1, ARFD1, RNF46
Gene Description
tripartite motif-containing 23
Omim ID
601747Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq
Other Designations
ADP-ribosylation factor domain protein 1|ADP-ribosylation factor domain protein 1, 64kDa|ARF domain protein 1|GTP-binding protein ARD-1|OTTHUMP00000123453|tripartite motif protein TRIM23
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