Protein Recombinant Marker is an accurate size protein molecular weight marker consisting of nine non-stained recombinant protein bands ranging from 10 KDa to 150 KDa. The recombinant proteins show sharp bands without heterogeneity derived from glycosylation, and a clear background allowing precise size determination. The 20 KDa and 80 KDa bands give higher intensity than other bands for easy identification of protein bands. The marker is supplied in gel loading buffer for direct loading onto SDS-PAGE without heating or adding a reducing agent.
50 mM Tris-HCl (pH 6.8), 2 mM EDTA, 1% SDS, 50 mM DTT, 10% glycerol
Store at -20°C (6 months at -20°C ), avoid repeated freezing and thawing.
There is no need to heat or add a reducing agent. Load 0.2-0.5 ul for silver staining.