ACTB polyclonal antibody
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More Files
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Western Blot
Western blot using ACTB polyclonal antibody (Cat # PAB9980) shows detection of a predominant band at ~42 KDa corresponding to ACTB (arrowhead) in various lysates.
Lysates are as follows : HEK 293 (Lane 2), human lung carcinoma A-549 (Lane 3) and mouse brain (Lane 4) all using the 800 nm channel (green).
Approximately 35 ug of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500.
Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Molecular weight markers are shown in lane 1 using the 700 nm channel (red).
IRDye™800 fluorescence image was captured using the Odyssey u Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
Immunofluorescence
Immunofluorescence staining of ACTB polyclonal antibody (Cat # PAB9980) at 1 : 200 is shown to detect actin at the neuromuscular junction of rana pipiens tissue (sections 4.2 um thick).
Personal Communication. S. Moeckel-Cole, Univ. MA, Amherst, MA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTB.
Immunogen
A synthetic peptide corresponding to amino acids 359-368 of human ACTB.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:1000-1:4000)
Immunofluorescence (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using ACTB polyclonal antibody (Cat # PAB9980) shows detection of a predominant band at ~42 KDa corresponding to ACTB (arrowhead) in various lysates.
Lysates are as follows : HEK 293 (Lane 2), human lung carcinoma A-549 (Lane 3) and mouse brain (Lane 4) all using the 800 nm channel (green).
Approximately 35 ug of each lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,500.
Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Molecular weight markers are shown in lane 1 using the 700 nm channel (red).
IRDye™800 fluorescence image was captured using the Odyssey u Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunofluorescence
Immunofluorescence staining of ACTB polyclonal antibody (Cat # PAB9980) at 1 : 200 is shown to detect actin at the neuromuscular junction of rana pipiens tissue (sections 4.2 um thick).
Personal Communication. S. Moeckel-Cole, Univ. MA, Amherst, MA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACTB
Entrez GeneID
60Protein Accession#
P60709;NP_001092Gene Name
ACTB
Gene Alias
PS1TP5BP1
Gene Description
actin, beta
Omim ID
102630Gene Ontology
HyperlinkGene Summary
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
Other Designations
PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|beta cytoskeletal actin
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Interactome
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Pathway
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Disease
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Publication Reference
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A role for beta-actin in RNA polymerase III transcription.
Hu P, Wu S, Hernandez N.
Genes & Development 2004 Dec; 18(24):2010.
Application：WB, Human, HeLa, IMR90 cells.
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Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12).
Dahlén A, Fletcher CD, Mertens F, Fletcher JA, Perez-Atayde AR, Hicks MJ, Debiec-Rychter M, Sciot R, Wejde J, Wedin R, Mandahl N, Panagopoulos I.
The American Journal of Pathology 2004 May; 164(5):1645.
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Proteomic identification of brain proteins that interact with dynein light chain LC8.
Navarro-Lerida I, Martinez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodriguez-Crespo I.
Proteomics 2004 Feb; 4(2):339.
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A role for beta-actin in RNA polymerase III transcription.
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