Goat polyclonal antibody raised against native APOA1.
Native purified human APOA1.
This antibody reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Quality Control Testing:
Antibody Reactive Against Native Purified Protein.
ELISA (1:10000-1:20000) Western Blot (1:5000-1:10000) Immunohistochemistry (1:50-1:200) The optimal working dilution should be determined by the end user.
In 125 mM Sodium Borate, 75 mM Sodium Chloride, 5 mM EDTA, pH 8.0 (0.01% Sodium Azide).
Store at 4°C. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical staining with APOA1 polyclonal antibody (Cat # PAB9966) was used at a 5 ug/mL to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq