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Last updated: 2014/4/21
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BIOtech
BULK-new

TFPI polyclonal antibody

  • Catalog # : PAB6017
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of TFPI.
  • Immunogen:
  • A synthetic peptide corresponding to human TFPI.
  • Sequence:
  • C-VKIAYEEIFVKNM
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 35
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform a (NP_006278.1) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • TFPI polyclonal antibody (Cat # PAB6017) staining (0.3 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein cells per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing TFPI and probed with TFPI polyclonal antibody (Cat # PAB6017) (mock transfection in first lane), tested by Origene.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 7035
  • Gene Name:
  • TFPI
  • Gene Alias:
  • EPI,LACI,TFI,TFPI1
  • Gene Description:
  • tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
  • Gene Summary:
  • This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000163472,anti-convertin,extrinsic pathway inhibitor,lipoprotein-associated coagulation inhibitor,tissue factor pathway inhibitor
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