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Last updated: 2014/4/14
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BIOtech
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KIT polyclonal antibody

  • Catalog # : PAB3359
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of KIT.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human KIT.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Monkey
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:100-500)
    Flow cytometry (1:10-50)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • The KIT polyclonal antibody (Cat # PAB3359) is used in Western blot to detect KIT in serum-starved HeLa cell lysate (lane 1) and primate testis tissue lysate (lane 2) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with KIT polyclonal antibody (Cat # PAB3359) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
  • ELISA
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using KIT polyclonal antibody (Cat # PAB3359)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 3815
  • Gene Name:
  • KIT
  • Gene Alias:
  • C-Kit,CD117,PBT,SCFR
  • Gene Description:
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • Gene Summary:
  • This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • mast/stem cell growth factor receptor,proto-oncogene tyrosine-protein kinase Kit,soluble KIT variant 1
  • Interactome
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