AKT3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The cell lysate derived from HeLa was immunoblotted by AKT3 polyclonal antibody (Cat # PAB12652) at 1 : 500.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT3.
Immunogen
A synthetic peptide corresponding to amino acids 120-132 of human AKT3.
Host
Rabbit
Theoretical MW (kDa)
56
Reactivity
Human
Specificity
This antibody only recognizes ~56 KDa of human AKT3, not AKT1 or AKT2. It also reacts with mouse and rat AKT3 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from HeLa was immunoblotted by AKT3 polyclonal antibody (Cat # PAB12652) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT3
Entrez GeneID
10000Gene Name
AKT3
Gene Alias
DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Gene Description
v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Omim ID
611223Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000037911|OTTHUMP00000037912|RAC-gamma serine/threonine protein kinase|protein kinase B gamma|serine threonine protein kinase, Akt-3|v-akt murine thymoma viral oncogene homolog 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines.
Nakatani K, Thompson DA, Barthel A, Sakaue H, Liu W, Weigel RJ, Roth RA.
The Journal of Biological Chemistry 1999 Jul; 274(31):21528.
Application：IP, WB-Ce, Human, 293T, MCF-7, T47D, MDA-MB-231, HBL-100 cells.
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Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines.
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