MYC-tag polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead).
Lane 1 contains ~35 ug of lysate from control 293T cells.
Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein.
Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC.
Western Blot (Recombinant protein)
MYC-tag polyclonal antibody (Cat # PAB10345) detects ~ 100 kDa carboxy terminal linked MYC-tagged recombinant protein present in ~35 ug of lysate by western blot (arrowhead).
Carboxy terminal linked MYC recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYC-tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 410-419 of human MYC-tag.
Sequence
EQKLISEEDL
Host
Rabbit
Reactivity
Human
Specificity
This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. This antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:135000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead).
Lane 1 contains ~35 ug of lysate from control 293T cells.
Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein.
Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC.Western Blot (Recombinant protein)
MYC-tag polyclonal antibody (Cat # PAB10345) detects ~ 100 kDa carboxy terminal linked MYC-tagged recombinant protein present in ~35 ug of lysate by western blot (arrowhead).
Carboxy terminal linked MYC recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYC
Entrez GeneID
4609Gene Name
MYC
Gene Alias
bHLHe39, c-Myc
Gene Description
v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
Other Designations
avian myelocytomatosis viral oncogene homolog|myc proto-oncogene protein|v-myc avian myelocytomatosis viral oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein phosphatase PPM1B inhibits DYRK1A kinase through dephosphorylation of pS258 and reduces toxic tau aggregation.
Ye Hyung Lee, Eunju Im, Minju Hyun, Joongkyu Park, Kwang Chul Chung.
The Journal of Biological Chemistry 2021 Jan; 296:100245.
Application：IP, WB-Tr, Human, Rat, H19-7, HEK 293, SH-SY5Y cells.
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Protein phosphatase PPM1B inhibits DYRK1A kinase through dephosphorylation of pS258 and reduces toxic tau aggregation.
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