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Last updated: 2015/4/19
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AKT1 (phospho S473) polyclonal antibody

  • Catalog # : PAB10323
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S473 of human AKT1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:15000-1:60000)
    Western Blot (1:200-1:1000)
    Immunohistochemistry (1:100-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Immunoblotting of AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1:200 dilution to detect phosphorylated AKT1 by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT1 kinase was used.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1 : 100 dilution to detect AKT1 by immunohistochemistry of formalin-fixed and paraffin embedded in (A) human breast tumor tissue, (B) a higher magnification and (C) normal human breast tissue of the breast tumor.
    The staining is much stronger than the weak basal level of phosphorylation in normal breast.
    AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used with no pretreatment of tissue.
    Signal was developed using Dako's Techmate streptavidin-biotin reagents.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence microscopy.
    Confocal image using AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) at a 1 : 40 dilution to detect phosphorylated AKT1 (green) in rat cardiomyocytes infected with adenovirus expressing with wild-type AKT1 in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 207
  • Protein Accession#:
  • P31749;AAH00479
  • Gene Name:
  • AKT1
  • Gene Alias:
  • AKT,MGC99656,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA
  • Gene Description:
  • v-akt murine thymoma viral oncogene homolog 1
  • Gene Summary:
  • The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
  • Other Designations:
  • RAC-alpha serine/threonine-protein kinase,murine thymoma viral (v-akt) oncogene homolog-1,protein kinase B,rac protein kinase alpha
  • Interactome
  • Interactome
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