Immunoblotting of AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1:200 dilution to detect phosphorylated AKT1 by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT1 kinase was used.
AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used at a 1 : 100 dilution to detect AKT1 by immunohistochemistry of formalin-fixed and paraffin embedded in (A) human breast tumor tissue, (B) a higher magnification and (C) normal human breast tissue of the breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) was used with no pretreatment of tissue. Signal was developed using Dako's Techmate streptavidin-biotin reagents.
Immunofluorescence microscopy. Confocal image using AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) at a 1 : 40 dilution to detect phosphorylated AKT1 (green) in rat cardiomyocytes infected with adenovirus expressing with wild-type AKT1 in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq