EGFR polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using EGFR polyclonal antibody (Cat # PAB10010) shows detection of aband at ~170 KDa (Lane 1) corresponding to human EGFR present in unstimulated (Lane 1) and EGF (50 ng/mL for 15 min) stimulated (Lane 2) A-431whole cell lysates (arrowhead).
Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocell-ulose.
After blocking the membrane was probedwith the primary antibody diluted to 1:1,000.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green).
Molecular weight estimationwas made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
Immunogen
A synthetic peptide corresponding to amino acids 1189-1199 of human EGFR.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000-1:32000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using EGFR polyclonal antibody (Cat # PAB10010) shows detection of aband at ~170 KDa (Lane 1) corresponding to human EGFR present in unstimulated (Lane 1) and EGF (50 ng/mL for 15 min) stimulated (Lane 2) A-431whole cell lysates (arrowhead).
Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocell-ulose.
After blocking the membrane was probedwith the primary antibody diluted to 1:1,000.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800nm channel, green).
Molecular weight estimationwas made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developedby LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGFR
Entrez GeneID
1956Protein Accession#
P00533;NP_005219Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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The EGF receptor provides an essential survival signal for SOS-dependent skin tumor development.
Sibilia M, Fleischmann A, Behrens A, Stingl L, Carroll J, Watt FM, Schlessinger J, Wagner EF.
Cell 2000 Jul; 102(2):211.
Application：WB-Ce, Mouse, Keratinocytes.
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Role of epidermal growth factor receptor and STAT-3 activation in autonomous proliferation of SUM-102PT human breast cancer cells.
Sartor CI, Dziubinski ML, Yu CL, Jove R, Ethier SP.
Cancer Research 1997 Mar; 57(5):978.
Application：WB-Ce, Human, SUM-1O2PT cells.
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Expression of ras oncogene p21 protein in normal and neoplastic ovarian tissues: correlation with histopathologic features and receptors for estrogen, progesterone, and epidermal growth factor.
Scambia G, Catozzi L, Panici PB, Ferrandina G, Coronetta F, Barozzi R, Baiocchi G, Uccelli L, Piffanelli A, Mancuso S.
Am J Obstet Gynecol 1993 Jan; 168(1 Pt 1):71.
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The EGF receptor provides an essential survival signal for SOS-dependent skin tumor development.
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