CD2 monoclonal antibody, clone UMCD2/IE7E8
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More Files
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Flow Cytometry
5 X 105 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80 uL of CD2 monoclonal antibody, clone UMCD2/IE7E8 (Cat # MAB6895) at 1 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.12 log 10 fluorescent units when compared to a Mouse IgG2a negative control.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD2.
Immunogen
Native purified CD2 from human rheumatoid synovial T cell line (ST-1) .
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80 uL of CD2 monoclonal antibody, clone UMCD2/IE7E8 (Cat # MAB6895) at 1 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.12 log 10 fluorescent units when compared to a Mouse IgG2a negative control. -
Gene Info — CD2
Entrez GeneID
914Gene Name
CD2
Gene Alias
SRBC, T11
Gene Description
CD2 molecule
Omim ID
186990Gene Ontology
HyperlinkGene Summary
sheep red blood cell receptor|OTTHUMP00000024366|OTTHUMP00000059318|lymphocyte-function antigen-2
Other Designations
CD2 antigen (p50), sheep red blood cell receptor|OTTHUMP00000024366|OTTHUMP00000059318|lymphocyte-function antigen-2
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Interactome
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Pathway
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Disease
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Publication Reference
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CD2 and the regulation of T cell anergy.
Bell GM, Imboden JB.
Journal of Immunology 1995 Sep; 155(6):2805.
Application：Flow Cyt, Human, Mouse, T cells.
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An anti-CD2 monoclonal antibody that both inhibits and stimulates T cell activation recognizes a subregion of CD2 distinct from known ligand-binding sites.
Kozarsky KF, Tsai C, Bott CM, Allada G, Li LL, Fox DA.
Cellular Immunology 1993 Sep; 150(2):235.
Application：Flow Cyt, WB-Ce, Human, PBMCs, Thymocytes.
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CD2 and the regulation of T cell anergy.
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