CD44 monoclonal antibody, clone MEM-263 (APC)
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More Files
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Western Blot (Tissue lysate)
Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263.
Lane 1 : lysate of human PBL.
Lane 2 : lysate of canine PBL.
Lane 3 : lysate of porcine PBL.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD44.
Immunogen
Native purified CD44 from African Green Monkey COS-7 cells.
Host
Mouse
Theoretical MW (kDa)
80-95
Reactivity
Dog, Human, Monkey, Pig
Specificity
This antibody reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 KDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells,etc.); it is negative on platelets and hepatocytes.
Form
Liquid
Conjugation
APC
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using CD44 monoclonal antibody, clone MEM-263.
Lane 1 : lysate of human PBL.
Lane 2 : lysate of canine PBL.
Lane 3 : lysate of porcine PBL.Flow Cytometry
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Gene Info — CD44
Entrez GeneID
960Gene Name
CD44
Gene Alias
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Gene Description
CD44 molecule (Indian blood group)
Omim ID
107269Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations
CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl
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Interactome
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Pathway
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Disease
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Publication Reference
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Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells.
Subramaniam V, Gardner H, Jothy S.
Experimental and Molecular Pathology 2007 Dec; 83(3):341.
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CD44 regulates cell migration in human colon cancer cells via Lyn kinase and AKT phosphorylation.
Subramaniam V, Vincent IR, Gardner H, Chan E, Dhamko H, Jothy S.
Experimental and Molecular Pathology 2007 May; 83(2):207.
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Down-regulation of CD44 contributes to the differentiation of HL-60 cells induced by ATRA or HMBA.
Liu J, Bi G, Wen P, Yang W, Ren X, Tang T, Xie C, Dong W, Jiang G.
Cellular & Molecular Immunology 2007 Feb; 4(1):59.
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Soluble CD44 secretion contributes to the acquisition of aggressive tumor phenotype in human colon cancer cells.
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