EGFR (phospho Y1173) monoclonal antibody, clone EM-13
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blotting analysis of EGFR (phospho-Tyr1173) by EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) in EGF-treated A-431 (Lane 1), CALU-3 (Lane 2), MCF-7 (Lane 3), Jurkat (Lane 4) and Ramos (Lane 5) cell lines (reduced conditions).
Immunoprecipitation
Immunoprecipitation of EGFR from EGF-treated A-431 cells by EGFR (phospho Y992) monoclonal antibody, clone EM-12 (Cat # MAB6476) (Lane 1), EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) (Lane 2). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
-
Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of EGFR.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y1173 of human EGFR.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with human EGFR (ErbB1/HER1) phosphorylated on tyrosine 1173.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry
Immunoprecipitation
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blotting analysis of EGFR (phospho-Tyr1173) by EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) in EGF-treated A-431 (Lane 1), CALU-3 (Lane 2), MCF-7 (Lane 3), Jurkat (Lane 4) and Ramos (Lane 5) cell lines (reduced conditions).Immunoprecipitation
Immunoprecipitation of EGFR from EGF-treated A-431 cells by EGFR (phospho Y992) monoclonal antibody, clone EM-12 (Cat # MAB6476) (Lane 1), EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) (Lane 2). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.Flow Cytometry
-
Gene Info — EGFR
Entrez GeneID
1956Gene Name
EGFR
Gene Alias
ERBB, ERBB1, HER1, PIG61, mENA
Gene Description
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Alterations in genes of the EGFR signaling pathway and their relationship to EGFR tyrosine kinase inhibitor sensitivity in lung cancer cell lines.
Gandhi J, Zhang J, Xie Y, Soh J, Shigematsu H, Zhang W, Yamamoto H, Peyton M, Girard L, Lockwood WW, Lam WL, Varella-Garcia M, Minna JD, Gazdar AF.
PLoS One 2009 Feb; 4(2):e4576.
-
Epidermal growth factor receptor activation protects gastric epithelial cells from Helicobacter pylori-induced apoptosis.
Yan F, Cao H, Chaturvedi R, Krishna U, Hobbs SS, Dempsey PJ, Peek RM Jr, Cover TL, Washington MK, Wilson KT, Polk DB.
Gastroenterology 2009 Jan; 136(4):1297.
-
EGFR-dependent migration of glial cells is mediated by reorganisation of N-cadherin.
Rappl A, Piontek G, Schlegel J.
Journal of Cell Science 2008 Dec; 121(Pt 24):4089.
-
Alterations in genes of the EGFR signaling pathway and their relationship to EGFR tyrosine kinase inhibitor sensitivity in lung cancer cell lines.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com