CD14 monoclonal antibody, clone biG 14
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Specification
Product Description
Mouse monoclonal antibody raised against CD14.
Immunogen
Monocytes of different species and immunoaffinity purified soluble human CD14.
Host
Mouse
Reactivity
Bovine, Dog, Guinea pig, Horse, Human, Pig
Specificity
biG 14 represents an excellent marker for CD14. CD14 has been shown to be involved in the LPS-induced cell activation. This antigen is present as a membrane bound form and as soluble antigen, both involved in cell stimulation by LPS. The plasma protein LBP plays an important role in this LPS-CD14 mediated cell activation. Monocytes and PMN are activated by the LBP-LPS complex via membrane CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for Endotoxin.
Form
Lyophilized
Isotype
IgG2a
Recommend Usage
Flow Cytometry (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at -20°C. For long term storage, please store at -80°C.
After reconstitution with 100 uL deionized water, store at -80°C. For long term storage, it is recommended to add 0.1% BSA.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — CD14
Entrez GeneID
929Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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Publication Reference
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Escherichia coli Braun lipoprotein induces a lipopolysaccharide-like endotoxic response from primary human endothelial cells.
Neilsen PO, Zimmerman GA, McIntyre TM.
The Journal of Immunology 2001 Nov; 167(9):5231.
Application:WB-Ce, Human, HUVECs.
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Binding of bacterial peptidoglycan to CD14.
Dziarski R, Tapping RI, Tobias PS.
The Journal of Biological Chemistry 1998 Apr; 273(15):8680.
Application:Added, Recombinant protein.
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Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli.
Stelter F, Bernheiden M, Menzel R, Jack RS, Witt S, Fan X, Pfister M, Schutt C.
European Journal of Biochemistry 1997 Jan; 243(1-2):100.
Application:Func, Flow Cyt, Human, Mouse, CHO cells, Human monocytes.
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Escherichia coli Braun lipoprotein induces a lipopolysaccharide-like endotoxic response from primary human endothelial cells.
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