CD14 monoclonal antibody, clone MEM-18 (FITC)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD14.
Immunogen
Native purified human CD14.
Host
Mouse
Theoretical MW (kDa)
53-55
Reactivity
Human, Non-Human Primates
Specificity
This antibody reacts with CD14, a 53-55 KDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by this antibody is located between amino acids 57-64.
Form
Liquid
Conjugation
FITC
Isotype
IgG1
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
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Gene Info — CD14
Entrez GeneID
929Gene Name
CD14
Gene Alias
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Gene Description
CD14 molecule
Omim ID
158120Gene Ontology
HyperlinkGene Summary
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq
Other Designations
CD14 antigen|monocyte differentiation antigen CD14
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Interactome
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Pathway
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Disease
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Publication Reference
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The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y.
Biochemical and Biophysical Research Communications 2005 Mar; 328(1):173.
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Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14.
Bazil V, Baudys M, Hilgert I, Stefanova I, Low MG, Zbrozek J, Horejsi V.
Molecular Immunology 1989 Jul; 26(7):657.
Application:Flow Cyt, Human, Human monocytes.
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Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen.
Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I.
European Journal of Immunology 1986 Dec; 16(12):1583.
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The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain.
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