MITF monoclonal antibody, clone, D5
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using MITF monoclonal antibody, clone, D5 (Cat # MAB11118) on formalin fixed, paraffin embedded human melanoma.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant protein of human MITF.
Immunogen
An N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
Host
Mouse
Reactivity
Dog, Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1
Recommend Usage
Gel Supershift Assay
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation (2 ug/mg of protein lysate)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
In Western blotting, it recognizes a doublet of 52-56 kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining using MITF monoclonal antibody, clone, D5 (Cat # MAB11118) on formalin fixed, paraffin embedded human melanoma.Immunohistochemistry (Frozen sections)
Immunoprecipitation
Gel Supershift Assay
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Gene Info — MITF
Entrez GeneID
4286Protein Accession#
O75030Gene Name
MITF
Gene Alias
MI, WS2A, bHLHe32
Gene Description
microphthalmia-associated transcription factor
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000195123|OTTHUMP00000195140|homolog of mouse microphthalmia
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Interactome
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Pathway
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Disease
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