CD44 monoclonal antibody, clone 8E2F3
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) against HeLa (1) and HUVE-12(2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa 628-699) recombinant protein (2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538).
Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence
Confocal immunofluorescence analysis of methanol-fixed A-431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry
Flow cytometric analysis of HeLa cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (A) and negative control (B).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CD44.
Immunogen
Recombinant protein corresponding to amino acids 628-699 of human CD44.
Host
Mouse
Theoretical MW (kDa)
82
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) against HeLa (1) and HUVE-12(2) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa 628-699) recombinant protein (2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538).Immunofluorescence
Confocal immunofluorescence analysis of PANC-1 cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Immunofluorescence
Confocal immunofluorescence analysis of methanol-fixed A-431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells using CD44 monoclonal antibody, clone 8E2F3 (Cat # MAB10538) (A) and negative control (B). -
Gene Info — CD44
Entrez GeneID
960Gene Name
CD44
Gene Alias
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Gene Description
CD44 molecule (Indian blood group)
Omim ID
107269Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq
Other Designations
CD44 antigen|CD44 antigen (homing function and Indian blood group system)|CDW44 antigen|GP90 lymphocyte homing/adhesion receptor|Hermes antigen|antigen gp90 homing receptor|cell adhesion molecule|cell surface glycoprotein CD44|chondroitin sulfate proteogl
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Interactome
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Disease
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Publication Reference
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miR-142-3p is a tumor suppressor that inhibits estrogen receptor expression in ER-positive breast cancer.
Behzad Mansoori, Ali Mohammadi, Morten F Gjerstorff, Solmaz Shirjang, Zahra Asadzadeh, Vahid Khaze, Uffe Holmskov, Tohid Kazemi, Pascal H G Duijf, Behzad Baradaran.
Journal of Cellular Physiology 2019 Sep; 234(9):16043.
Application：WB-Tr, Human, MCF-7 cells.
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miR-142-3p is a tumor suppressor that inhibits estrogen receptor expression in ER-positive breast cancer.
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