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Western Blot (Cell lysate)

Identification of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) by Western blotting. Using crude cell extracts of MCF-7 untreated (lane 1) and treated with nocodazoleat 100 ng/ml for 48 h (lane2).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) on formalin-fixed paraffin-embedded human prostate.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) on formalin-fixed paraffin-embedded human stomach.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of TP53 (phospho-S315) monoclonal antibody, clone 18 (Cat # MAB0660) on formalin-fixed paraffin-embedded human ovary.

Immunofluorescence

Immunofluorescence staining of p53 phosho-Ser315 in nuclei of HeLa cells subjected DNA damage.
1. HeLa cells were treated with 100 nM Doxorubicin for 24 hr, fixed with 4% paraformaldehyde overnight, permealized with 0.25% Triton X-100 in PBS for 10 min. 2. Incubate cells with 1.5% BSA in PBS for 30 min to block unspecific binding of the antibodies. Incubate the cells with 1/1,000 diluted TP53 (phospho S315) monoclonal antibody, clone 18 (Cat # MAB0660) in 1% BSA in PBS at 4°C overnight. Incubate cells with the secondary antibody, goat anti-mouse IgG conjugated with Alex 488, at 1/1,000 dilution in 1% BSA for 1 hr at room temperature. 4. Nucleus (DNA) was stained with DAPI.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

7157

Protein Accession#

P04637

Gene Name

TP53

Gene Alias

FLJ92943, LFS1, TRP53, p53

Gene Description

tumor protein p53

Omim ID

114480 114500 114550 151623 161550 191170 202300 260350

Gene Ontology

Hyperlink

Gene Summary

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq

Other Designations

p53 antigen|p53 transformation suppressor|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53