ACAD8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ACAD8 transfected lysate.
Western Blot
Lane 1: ACAD8 transfected lysate ( 45.76 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACAD8 full-length
Host
Human
Theoretical MW (kDa)
45.76
Interspecies Antigen Sequence
Mouse (89); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACAD8 antibody (H00027034-B01) by Western Blots.
SDS-PAGE Gel
ACAD8 transfected lysate.
Western Blot
Lane 1: ACAD8 transfected lysate ( 45.76 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACAD8
Entrez GeneID
27034GeneBank Accession#
BC001964.1Protein Accession#
AAH01964.1Gene Name
ACAD8
Gene Alias
ACAD-8, FLJ22590
Gene Description
acyl-Coenzyme A dehydrogenase family, member 8
Gene Ontology
HyperlinkGene Summary
Isobutyryl-CoA dehydrogenase (EC 1.3.99), encoded by the ACAD8 gene, catalyzes the third step of the degradation of the branched chain amino acid valine (Nguyen et al., 2002 [PubMed 12359132]).[supplied by OMIM
Other Designations
acyl-coenzyme A dehydrogenase 8
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Interactome
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Pathway
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Disease
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