TOP3B monoclonal antibody (M01), clone 4F11
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More Files
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Western Blot (Cell lysate)
TOP3B monoclonal antibody (M01), clone 4F11 Western Blot analysis of TOP3B expression in K-562 ( Cat # L009V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.
Lane 1: TOP3B transfected lysate(96.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (120.56 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TOP3B.
Immunogen
TOP3B (AAH02432, 1 a.a. ~ 862 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWANTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (120.56 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TOP3B monoclonal antibody (M01), clone 4F11 Western Blot analysis of TOP3B expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.
Lane 1: TOP3B transfected lysate(96.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — TOP3B
Entrez GeneID
8940GeneBank Accession#
BC002432Protein Accession#
AAH02432Gene Name
TOP3B
Gene Alias
FLJ39376
Gene Description
topoisomerase (DNA) III beta
Omim ID
603582Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described. [provided by RefSeq
Other Designations
topoisomerase III beta
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Interactome
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Pathway
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