TWIST1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TWIST1 transfected lysate.
Western Blot
Lane 1: TWIST1 transfected lysate ( 21.00 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TWIST1 full-length
Host
Human
Theoretical MW (kDa)
21
Interspecies Antigen Sequence
Mouse (93); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TWIST1 antibody (H00007291-D01P) by Western Blots.
SDS-PAGE Gel
TWIST1 transfected lysate.
Western Blot
Lane 1: TWIST1 transfected lysate ( 21.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TWIST1
Entrez GeneID
7291GeneBank Accession#
NM_000474.3Protein Accession#
NP_000465.1Gene Name
TWIST1
Gene Alias
ACS3, BPES2, BPES3, SCS, TWIST, bHLHa38
Gene Description
twist homolog 1 (Drosophila)
Gene Ontology
HyperlinkGene Summary
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq
Other Designations
B-HLH DNA binding protein|H-twist|TWIST homolog of drosophila|twist
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Interactome
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Disease
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