TWIST1 monoclonal antibody (M03), clone 2F8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M03), clone 2F8.
Lane 1: TWIST1 transfected lysate(21 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TWIST1 is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to TWIST1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.07 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TWIST1.
Immunogen
TWIST1 (NP_000465, 100 a.a. ~ 202 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.07 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TWIST1 expression in transfected 293T cell line by TWIST1 monoclonal antibody (M03), clone 2F8.
Lane 1: TWIST1 transfected lysate(21 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TWIST1 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TWIST1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — TWIST1
Entrez GeneID
7291GeneBank Accession#
NM_000474Protein Accession#
NP_000465Gene Name
TWIST1
Gene Alias
ACS3, BPES2, BPES3, SCS, TWIST, bHLHa38
Gene Description
twist homolog 1 (Drosophila)
Gene Ontology
HyperlinkGene Summary
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq
Other Designations
B-HLH DNA binding protein|H-twist|TWIST homolog of drosophila|twist
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Interactome
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Disease
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