TRIM21 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: TRIM21 transfected lysate ( 54.2 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
TRIM21 transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TRIM21 full-length
Host
Human
Theoretical MW (kDa)
52.36
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TRIM21 antibody (H00006737-B01) by Western Blots.
Western Blot
Lane 1: TRIM21 transfected lysate ( 54.2 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
TRIM21 transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TRIM21
Entrez GeneID
6737GeneBank Accession#
NM_003141Protein Accession#
NP_003132Gene Name
TRIM21
Gene Alias
RNF81, RO52, SSA, SSA1
Gene Description
tripartite motif-containing 21
Omim ID
109092Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq
Other Designations
Sicca syndrome antigen A|Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)|tripartite motif protein 21
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Interactome
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Disease
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