TRIM21 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of TRIM21 transfected lysate using rabbit polyclonal anti-TRIM21 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-TRIM21.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of TRIM21 transfected lysate using rabbit polyclonal anti-TRIM21 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-TRIM21.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-TRIM21 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-TRIM21 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — TRIM21
Entrez GeneID
6737Gene Name
TRIM21
Gene Alias
RNF81, RO52, SSA, SSA1
Gene Description
tripartite motif-containing 21
Omim ID
109092Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq
Other Designations
Sicca syndrome antigen A|Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)|tripartite motif protein 21
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Interactome
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Pathway
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Disease
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